The Best Homemade Beef Jerky Recipe

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This is the best homemade beef jerky recipe, and it’s easy to make without any special equipment.

Beef jerky on a wooden surface.

After road tripping with my family and sampling jerky from gas stations along the way — and spending a small fortune on it — I resolved to come up with a good homemade beef jerky recipe. The good news is that beef jerky is surprisingly easy to make and doesn’t require any special equipment other than a standard oven, baking sheets, and wire racks. However, most homemade jerky isn’t nearly as tender as the store-bought kind. That’s because commercial jerky producers use special equipment and curing preservatives to make their signature jerky. Finally, after falling down an internet rabbit hole of pitmaster video tutorials, I learned the secret to making tender jerky at home: adding plenty of sugar to the marinade. The extra sugar not only helps preserve the meat but also locks in moisture. This recipe makes a salty-sweet, smoky jerky with a chewy yet tender texture, similar to the well-known brands.

What You’ll Need To Make Homemade Beef Jerky

Jerky ingredients including Worcestershire sauce, soy sauce, and pepper.When making beef jerky, it’s important to start with a well-trimmed, lean cut of meat, as fat does not dry out and accelerates spoilage. An eye of round roast is ideal; it’s affordable, accessible, lean, and easy to trim. Before slicing, pop it in the freezer for 1 to 2 hours; it will be much easier to cut.

The marinade contains soy sauce, brown sugar, Worcestershire sauce, seasoning, and unseasoned meat tenderizer. Meat tenderizer contains bromelain, an enzyme that breaks down meat tissue. You can find it in the spice section of your supermarket (I use McCormick).

Step-by-Step Instructions

1. Slice the Meat

slicing the meat for beef jerky
Slice the meat between 1/8 and 1/4 inch thick with the grain. If the roast is too thick to slice easily, cut it in half horizontally before slicing.
slicing the meat

2. Make the Marinade

In a medium bowl, combine the brown sugar, soy sauce, Worcestershire sauce, smoked paprika, meat tenderizer, black pepper, red pepper flakes, onion powder, and garlic powder.

marinade for beef jerky

Whisk until evenly combined and the sugar is dissolved.

Bowl of whisked marinade.

3. Marinate the Beef

Add the meat to the marinade and toss until all of the pieces are evenly coated. Cover with plastic wrap (or transfer to a large ziplock bag) and marinate in the refrigerator for at least 12 hours or overnight. Toss the meat (or flip the bag) once or twice to be sure the meat marinates evenly.

marinate the beef

4. Dry Out The Meat

Line two baking sheets with aluminum foil for easy clean-up. Place a wire rack over each pan. Preheat the oven to 175°F and set two oven racks in the centermost positions.
Arrange the marinated meat on the wire racks in a single layer.

marinated beef on wire rack

Bake, rotating the pans from front to back and top to bottom midway through, until the meat is dried out, 3 to 4 hours. To determine if the jerky is thoroughly dried out, take a piece out of the oven and let it cool to room temperature. It should be dry to the touch, leather-like in appearance, and chewy but still somewhat tender.
cooked beef jerky

Store the jerky inside an airtight plastic container, Ziploc bag, or airtight glass jars. Properly dried jerky will keep at room temperature for about one week.

video tutorial

Beef jerky on a wooden surface.

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The Best Homemade Beef Jerky Recipe

This is the best homemade beef jerky recipe, and it’s easy to make without any special equipment.

Servings: 12 to 14 servings
Prep Time: 30 Minutes
Cook Time: 3 Hours
Total Time: 3 Hours 30 Minutes, plus at least 12 hours to marinate

Ingredients

  • One 3-pound eye of round roast (see note), trimmed of fat and silver skin
  • 1 cup (packed) dark brown sugar
  • 1 cup soy sauce
  • 3 tablespoons Worcestershire sauce
  • 1 tablespoon smoked paprika
  • 1 teaspoon unseasoned meat tenderizer (see note)
  • 1 teaspoon freshly ground black pepper
  • 1 teaspoon red pepper flakes
  • 1 teaspoon onion powder
  • ½ teaspoon garlic powder

Instructions

  1. Slice the meat between ⅛ and ¼ inch thick with the grain. (If the roast is too thick to slice easily, cut it in half horizontally before slicing.)
  2. Make the marinade: In a medium bowl, combine the brown sugar, soy sauce, Worcestershire sauce, smoked paprika, meat tenderizer, black pepper, red pepper flakes, onion powder, and garlic powder. Whisk until evenly combined and the sugar is dissolved.
  3. Add the meat to the marinade and toss until all of the pieces are evenly coated. Cover with plastic wrap or transfer to a large ziplock bag and marinate in the refrigerator for at least 12 hours or overnight. Toss the meat (or flip the bag) once or twice to be sure the meat marinates evenly.
  4. Line two baking sheets with aluminum foil for easy clean-up. Place a wire rack over each pan. Preheat the oven to 175°F and set two oven racks in the centermost positions.
  5. Arrange the marinated meat on the wire racks in a single layer. Bake, rotating the pans from front to back and top to bottom midway through, until the meat is dried out, 3 to 4 hours. To determine if the jerky is thoroughly dried out, take a piece out of the oven and let it cool to room temperature. It should be dry to the touch, leather-like in appearance, and chewy but still somewhat tender.
  6. Store the jerky inside an airtight plastic container, Ziploc bag, or airtight glass jars. Properly dried jerky will keep at room temperature for about one week. Refrigerate or freeze for longer storage.
  7. Note: Pop the meat in the freezer for 1 to 2 hours before slicing; it will be easier to cut.
  8. Note: Meat tenderizer contains bromelain, an enzyme that breaks down meat tissue. You can find it in the spice section of your supermarket. (I use McCormick.)

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Comments

  • What an outstanding recipe !!! The results are far superior to commercial jerky available in stores. I have already shared this with friends and family who are jerky-aficionados. Well done.

  • Hi, made this with moosemeat and it was pretty darn good! Did not use meat tenderizer (mostly because forgot to get it at store). Used the same temperature setting but did convection bake and cooked for 2.5 hours, it was chewy but not tough. Added a dash of worcestershire and mustard, as to my personal taste.

  • Wow, Thank you for the recipe and all the important tips/explanation included. I just bought over $100 worth of beef jerky because my husband is cutting down carb for his snacks and he loves beef jerky. I love Taiwanese jerky which they use both pork and beef as the meat source. I am going to use fresh pineapple to add to the marinade since I don’t have bromelain on hand. I have two questions? Is there a difference in final meat quality if eye of round instead of round is used? I believe eye of round is a more expensive cut. Wonder if it’s worth the extra expense.
    To do: I will try to use pork for this recipe. I have a vacuum sealer and I just found another good use of my sealer!

    • Hi Janet, I’ve tried several different cuts of meat, and I had the best results with eye of round, so I’d recommend sticking with that for the best results. (That said, I haven’t tried it with round.)

  • Made this yesterday, it is fantastic!! I made it for my husband and sons, tired of buying expensive, full-of-preservatives store bought jerky. Now I’m in trouble because they said from now on they only want this!!!!
    Any thoughts on if I try to 1.5 or double the recipe (yesterday’s single batch is nearly gone already!!) would I need to change oven heat or time? Or both?

    Another awesome recipe, every single one I’ve tried off this site has been a winner!!

    • So glad all your boys enjoyed it, Katie! It doesn’t last long at my house either. You can definitely increase/double the recipe. I would keep the oven temperature the same but allow extra time in the oven…I’m guessing an additional 30-60 minutes but just keep an eye on it (and be sure to rotate all the pans in the oven so they cook evenly).

  • Hi Jenn

    I made the homemade jerky. It was absolutely delicious.

    I decreased the amount of brown sugar to 1/2 cup and instead of using the oven the dehydrator did a great job. Dehydrate according to instrutions.

    Sue Price

    • how long did you put it in the dehydrator for?

      • — Maison parker on July 15, 2023
      • Reply
  • As always with your recipes, great flavor. I did marinate the beef for 2 days. Good snack alternative for those cookies we have been snacking on 🙂

  • Hi Jenn:

    I love all your recipes especially the Secret Chocolate cookies. So good. Sometimes I put more processed oats in it for the texture.

    Also enjoyed making the almond biscotti too. What a treat!

    I tried your beef jerky but found it to be a bit tough. What did I do wrong?

    Please advise me. Many thanks

    Judy Knight

    • Hi Judy, Beef jerky is chewy by nature, but this version shouldn’t be tough. Did you by chance make any substitutions or use a different cut of meat?

  • Although I’ve never been a beef jerky fan, I made your recipe for my husband, who claims it’s the best he’s ever had. I may soon be eating jerky too. Thanks for a great recipe!

  • I made this beef jerky exactly as shown except for adding a teaspoon of baking soda in place of meat tenderizer. As with other recipes found on Once Upon a Chef, this one turned out great! I will definitely be making beef jerky again, probably sooner rather than later, since I find it difficult to just eat a slice or two at one time. Thanks for the recipe Jenn.

  • Beef jerky is a classic for Road trips and camping- Usually store bought ones are a bit too sweet or hard. This one is nothing like that- perfect amount of chewiness and sweetness, makes everyone want to keep eating! All of Jen’s tips and tricks for almost every recipe leads to nothing but the BEST food ever. From my experience of being Jenn’s #1 true fan, I can tell you one thing: She never leaves us hanging- only shares recipes that are nothing short from perfect.

    • — Hannah Sebastian
    • Reply

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